Metabolome-wide association study of the relationship between chlorpyrifos exposure and first trimester serum metabolite levels in pregnant Thai farmworkers

• Published in: Environmental research 2022-12, Vol.215 (Pt 2), p.114319-114319, Article 114319
• Main Authors: Liang, Donghai, Batross, Jonathan, Fiedler, Nancy, Prapamontol, Tippawan, Suttiwan, Panrapee, Panuwet, Parinya, Naksen, Warangkana, Baumert, Brittney O., Yakimavets, Volha, Tan, Youran, D'Souza, Priya, Mangklabruks, Ampica, Sittiwang, Supattra, Kaewthit, Kristsanachai, Kohsuwan, Kanyapak, Promkam, Nattawadee, Pingwong, Sureewan, Ryan, P. Barry, Barr, Dana Boyd, Steenland, Kyle, Smarr, Melissa M., Lee, Grace, Yakimavets, Olya, Hawkins, Tamaria, Sullivan, Margaret, Ohman-Strickland, Pamela, Robson, Mark, Iamsupasit, Sompoch, Siriwong, Wattasit, Barr, Kathryn J., Greenwald, Rachel, Cheatham, Carol, Mangklapuark, Ampica, Kerdnoi, Tanyaporn, Srirak, Namtip, Hongsibsong, Surat, Dokjunyam, Chayada, Nimmapirat, Pimjuta, Promduang, Wathoosiri, Jayakittivaraloes, Natabhol, Kunsupa, Kewalin, Kantasri, Anchana, Kaewthit, Kritsanachai, Wongkampaun, Anchalee, Thongjan, Nathaporn, Dawandee, Ranuka, Chaimidchid, Maytinee, Soonsawat, Sasiwimon, Sritongkom, Kingpaka, Namakunna, Sakawrat, Pinasu, Soythong
• Format: Article
• Language: English
• Subjects: Biomarkers; Biomarkers - urine; Birth cohort; Child; Chlorpyrifos; Creatinine; Cystine - metabolism; Cytochromes - metabolism; Farmers; Farmworker; Fatty Acids; Female; Glutathione - metabolism; Humans; Insecticides - toxicity; Metabolome; Metabolomics; NAD - metabolism; Niacinamide; Organophosphate; Organophosphorus Compounds - toxicity; Pesticides - urine; Pregnancy; Pregnancy Trimester, First; Reactive Oxygen Species; Thailand; Tryptophan - metabolism
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2022.114319

Organophosphate (OP) insecticides, including chlorpyrifos, have been linked with numerous harmful health effects on maternal and child health. Limited data are available on the biological mechanisms and endogenous pathways underlying the toxicity of chlorpyrifos exposures on pregnancy and birth outcomes. In this study, we measured a urinary chlorpyrifos metabolite and used high-resolution metabolomics (HRM) to identify biological perturbations associated with chlorpyrifos exposure among pregnant women in Thailand, who are disparately exposed to high levels of OP insecticides. This study included 50 participants from the Study of Asian Women and their Offspring's Development and Environmental Exposures (SAWASDEE). We used liquid chromatography-high resolution mass spectrometry to conduct metabolic profiling on first trimester serum samples collected from participants to evaluate metabolic perturbations in relation to chlorpyrifos exposures. We measured 3,5,6-trichloro-2-pyridinol (TCPy), a specific metabolite of chlorpyrifos and chlorpyrifos-methyl, in first trimester urine samples to assess the levels of exposures. Following an untargeted metabolome-wide association study workflow, we used generalized linear models, pathway enrichment analyses, and chemical annotation to identify significant metabolites and pathways associated with urinary TCPy levels. In the 50 SAWASDEE participants, the median urinary TCPy level was 4.36 μg TCPy/g creatinine. In total, 691 unique metabolic features were found significantly associated with TCPy levels (p