Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly Anastrepha ludens

spp. (Diptera: Tephritidae) infestations cause significant economic losses in commercial fruit production worldwide. However, some plants quickly counteract the insertion of eggs by females by generating neoplasia and hindering eclosion, as is the case for Mill., cv. Hass (Hass avocados). We followe...

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Published in:International journal of molecular sciences 2023-01, Vol.24 (3), p.2060
Main Authors: Aluja, Martín, Vázquez-Rosas-Landa, Mirna, Cerqueda-García, Daniel, Monribot-Villanueva, Juan L, Altúzar-Molina, Alma, Ramírez-Vázquez, Mónica, Velázquez-López, Olinda, Rosas-Saito, Greta, Alonso-Sánchez, Alexandro G, Ortega-Casas, Rafael, Enríquez-Valencia, Adrián José, Guerrero-Analco, José A, Ibarra-Laclette, Enrique
Format: Article
Language:English
Subjects:
Anastrepha ludens
Animals
Avocados
Biosynthesis
Cell walls
Chitin
Eclosion
Economic impact
Eggs
Evaluation
Female
Females
Fruit
Fruits
Herbivores
Hypersensitive response
Insects
Magnoliopsida
Metabolites
Metabolomics
Molecular modelling
Oviposition
Pathogens
Persea
Phytoalexins
Polyphenols
Tephritidae - genetics
Transcriptomics
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Summary:spp. (Diptera: Tephritidae) infestations cause significant economic losses in commercial fruit production worldwide. However, some plants quickly counteract the insertion of eggs by females by generating neoplasia and hindering eclosion, as is the case for Mill., cv. Hass (Hass avocados). We followed a combined transcriptomics/metabolomics approach to identify the molecular mechanisms triggered by Hass avocados to detect and react to the oviposition of the pestiferous (Loew). We evaluated two conditions: fruit damaged using a sterile pin (pin) and fruit oviposited by females (ovi). We evaluated both of the conditions in a time course experiment covering five sampling points: without treatment (day 0), 20 min after the treatment (day 1), and days 3, 6, and 9 after the treatment. We identified 288 differentially expressed genes related to the treatments. Oviposition (and possibly bacteria on the eggs' surface) induces a plant hypersensitive response (HR), triggering a chitin receptor, producing an oxidative burst, and synthesizing phytoalexins. We also observed a process of cell wall modification and polyphenols biosynthesis, which could lead to polymerization in the neoplastic tissue surrounding the eggs.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24032060