OsGLP3‐7 positively regulates rice immune response by activating hydrogen peroxide, jasmonic acid, and phytoalexin metabolic pathways

• Published in: Molecular plant pathology 2023-03, Vol.24 (3), p.248-261
• Main Authors: Sun, Bingrui, Li, Wenyan, Ma, Yamei, Yu, Ting, Huang, Wenjie, Ding, Jierong, Yu, Hang, Jiang, Liqun, Zhang, Jing, Lv, Shuwei, Yang, Jianyuan, Yan, Shijuan, Liu, Bin, Liu, Qing
• Format: Article
• Language: English
• Subjects: Abscisic acid; Accumulation; Biosynthesis; Blight; Cytoplasm; Disease resistance; Experiments; Flowers & plants; Gene expression; Genes; Genetic engineering; Genetically modified plants; germin‐like protein; H2O2; Health aspects; Hydrogen peroxide; Immune response; Infections; Inoculation; Jasmonic acid; Kinases; Leaves; Localization; Metabolic pathways; Metabolism; Metabolites; Original; Pathogens; Physiological aspects; Phytoalexin; Phytoalexins; Plant diseases; Plant genetics; Plant resistance; Plants; Proteins; Rice; Rice blast; RNA-mediated interference; Roles; Superoxide dismutase; Transgenic plants
• ISSN: 1464-6722; 1364-3703
• DOI: 10.1111/mpp.13294

Although germin‐like proteins (GLPs) have been demonstrated to participate in plant biotic stress responses, their specific functions in rice disease resistance are still largely unknown. Here, we report the identification and characterization of OsGLP3‐7, a member of the GLP family in rice. Expression of OsGLP3‐7 was significantly induced by pathogen infection, jasmonic acid (JA) treatment, and hydrogen peroxide (H2O2) treatment. OsGLP3‐7 was highly expressed in leaves and sublocalized in the cytoplasm. Overexpression of OsGLP3‐7 increased plant resistance to leaf blast, panicle blast, and bacterial blight, whereas disease resistance in OsGLP3‐7 RNAi silenced plants was remarkably compromised, suggesting this gene is a positive regulator of disease resistance in rice. Further analysis showed that OsGLP3‐7 has superoxide dismutase (SOD) activity and can influence the accumulation of H2O2 in transgenic plants. Many genes involved in JA and phytoalexin biosynthesis were strongly induced, accompanied with elevated levels of JA and phytoalexins in OsGLP3‐7‐overexpressing plants, while expression of these genes was significantly suppressed and the levels of JA and phytoalexins were reduced in OsGLP3‐7 RNAi plants compared with control plants, both before and after pathogen inoculation. Moreover, we showed that OsGLP3‐7‐dependent phytoalexin accumulation may, at least partially, be attributed to the elevated JA levels observed after pathogen infection. Taken together, our results indicate that OsGLP3‐7 positively regulates rice disease resistance by activating JA and phytoalexin metabolic pathways, thus providing novel insights into the disease resistance mechanisms conferred by GLPs in rice. The germin‐like protein OsGLP3‐7 positively regulates rice resistance to leaf blast, panicle blast, and bacterial blight, mediating the activation of the hydrogen peroxide, jasmonic acid, and phytoalexin metabolic pathways in rice.