Direct evidence of fiber-protein-directed hemagglutination by canine adenoviruses

Canine adenoviruses (CAdVs) are divided into two serotypes, CAdV1 and CAdV2, whose members mainly cause infectious hepatitis and laryngotracheitis, respectively, in canids. To gain insight into the molecular basis of viral hemagglutination, we constructed chimeric viruses whose fiber proteins or the...

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Bibliographic Details
Published in:Archives of virology 2023-03, Vol.168 (3), p.93-93, Article 93
Main Authors: Matsugo, Hiromichi, Kamiki, Haruhiko, Ishida, Hiroho, Kobayashi-Kitamura, Tomoya, Takenaka-Uema, Akiko, Murakami, Shin, Horimoto, Taisuke
Format: Article
Language:English
Subjects:
Adenoviridae
Adenoviruses
Adenoviruses, Canine - genetics
Adenoviruses, Human - genetics
Amino Acid Sequence
Artificial chromosomes
Biomedical and Life Sciences
Biomedicine
Brief Report
Capsid Proteins - metabolism
Hemagglutination
Hepatitis
Infectious Diseases
Laboratories
Laryngotracheitis
Life sciences
Medical Microbiology
Proteins
Serotypes
Virology
Virus attachment
Viruses
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Summary:Canine adenoviruses (CAdVs) are divided into two serotypes, CAdV1 and CAdV2, whose members mainly cause infectious hepatitis and laryngotracheitis, respectively, in canids. To gain insight into the molecular basis of viral hemagglutination, we constructed chimeric viruses whose fiber proteins or their knob domains, which play a role in viral attachment to cells, were swapped among CAdV1, CAdV2, and bat adenovirus via reverse genetics. The results revealed that, in each case, viral hemagglutination was specifically mediated by the fiber protein or knob domain, providing direct evidence for fiber-protein-directed receptor-binding characteristics of CAdVs.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-023-05718-5