Protective efficacy of dietary natural antioxidants on microplastic particles-induced histopathological lesions in African catfish (Clarias gariepinus)

• Published in: Environmental science and pollution research international 2023-02, Vol.30 (9), p.24424-24440
• Main Authors: Sayed, Alaa El-Din H., Hana, Mervat N., Hamed, Mohamed, Abdel-Latif, Hany M. R., Lee, Jae-Seong, Soliman, Hamdy A. M.
• Format: Article
• Language: English
• Subjects: Animals; antioxidants; Antioxidants - pharmacology; apoptosis; aquaculture; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; biomarkers; blood; catfish; Catfishes - physiology; Chlorella; citric acid; Clarias gariepinus; cytoplasm; Diet; dissociation; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; glycogen; hemorrhage; hepatocytes; histology; histopathology; hypertrophy; ingestion; intestines; kidneys; Liver; lycopene; Lycopene - pharmacology; Microplastics; muscles; necrosis; Plastics; pollutants; Research Article; shrinkage; toxicity; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 1614-7499; 0944-1344; 1614-7499
• DOI: 10.1007/s11356-022-23789-w

Microplastic particles (MPs) are a common environmental pollutant easily ingested by fish in aquaculture. The current study evaluated the protective efficacies of some antioxidant, e.g., lycopene, citric acid, and chlorella, against the toxic effects of MP ingestion by Clarias gariepinus using histopathological biomarkers. Five experimental groups were established, a control group receiving only a standard diet, a group exposed to 500 mg/kg MP concomitant with the standard diet, and three antioxidant groups exposed to MPs plus either lycopene (500 mg/kg), citric acid (30 g/kg), or chlorella (50 g/kg) in the standard diet. After 15 days, fish were sacrificed for histological and histochemical examinations. Histological analysis of the kidney for group 2 (fed 500 mg/kg MPs alone) revealed distributed tissue dissociation, regional glomerular hypertrophy or shrinkage, melanomacrophage accumulation, and expansion of Bowman’s space, while liver tissue exhibited dilation and rupture of the central vein wall, hemorrhage, cytoplasmic vacuolation, and cellular necrosis or apoptosis. Fish exposed to MPs also exhibited connective tissue fiber accumulation around renal blood vessels, renal tubules, the central hepatic vein, hepatic blood sinusoids, and serosal, muscle, and submucosal layers of the intestine. In addition, MP exposure reduced carbohydrate (mainly glycogen) contents in the brush borders and basement membranes of renal tubules, glomeruli, and intestinal tissues as well as in the cytoplasm of hepatocytes. These signs of renal, hepatic, and intestinal histopathology were fully or partially reversed by dietary lycopene, chlorella, or citric acid. Enhancing dietary antioxidants is an effective strategy for preventing MP toxicity in Clarias gariepinus in aquaculture.