Evaluation of phenotypic detection of carbapenemase-producing Pseudomonas spp. from clinical isolates

• Published in: Brazilian journal of microbiology 2023-03, Vol.54 (1), p.135-141
• Main Authors: de Oliveira Santos, Ivson Cassiano, da Conceiçāo Neto, Orlando Carlos, da Costa, Bianca Santos, Teixeira, Camila Bastos Tavares, da Silva Pontes, Leilane, Silveira, Melise Chaves, Rocha-de-Souza, Cláudio Marcos, Carvalho-Assef, Ana Paula D’Alincourt
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Antibiotics; Bacteria; Bacterial Proteins - pharmacology; beta-Lactamases; Biomedical and Life Sciences; Carbapenemase; Carbapenems; Clinical isolates; Clinical Microbiology - Research Paper; Edetic acid; Edetic Acid - pharmacology; Epidemiology; Ethylenediaminetetraacetic acids; Food Microbiology; Gram-negative bacteria; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbial Sensitivity Tests; Microbiology; Multidrug resistance; Mycology; Performance evaluation; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas putida; Sensitivity; Sensitivity and Specificity
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-022-00857-4

Carbapenems are considered last-resort antibiotics for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. Although the main mechanism of carbapenem-resistance in Pseudomonas aeruginosa is the loss of OprD porin, carbapenemases continue to be a problem worldwide. The aim of this study was to evaluate the performance of phenotypic tests (Carba NP, Blue Carba, and mCIM/eCIM) for detection of carbapenemase-producing Pseudomonas spp. in Brazil. One hundred twenty-seven Pseudomonas spp. clinical isolates from different Brazilian states were submitted to phenotypic and molecular carbapenemase detection. A total of 90 carbapenemase-producing P. aeruginosa and 5 Pseudomonas putida (35, bla VIM-2 ; 17, bla SPM-1 ; 2, bla IMP-10 ; 1, bla VIM-24 ; 1, bla NDM-1 ; 39, bla KPC-2 ). The phenotypic Carba NP, Blue Carba, and mCIM/eCIM showed sensitivity of 94.7%, 93.6%, and 93.6%, and specificity of 90.6%, 100%, and 96.8%, respectively. However, only the Carba NP presented the highest sensitivity and showed the ability in differentiating the carbapenemases between class A and class B using EDTA. Blue Carba failed to detect most of the class B carbapenemases, having the worst performance using EDTA. Our results show changes in the epidemiology of the spread of carbapenemases and the importance of their detection by phenotypic and genotypic tests. Such, it is essential to use analytical tools that faithfully detect bacterial resistance in vitro in a simple, sensitive, rapid, and cost-effective way. Much effort must be done to improve the current tests and for the development of new ones.