ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines

A limited number of studies are devoted to regulating expression in cancer. Hence, we aimed to unveil the regulation of expression in MCF-7 breast cancer cells (with high expression) and taxane-resistant MCF-7 sublines (manifesting even higher expression). We found that transcription is regulated pr...

Full description

Saved in:
Bibliographic Details
Published in:Genes 2023-01, Vol.14 (2), p.296
Main Authors: Daniel, Petr, Balušíková, Kamila, Václavíková, Radka, Šeborová, Karolína, Ransdorfová, Šárka, Valeriánová, Marie, Wei, Longfei, Jelínek, Michael, Tlapáková, Tereza, Fleischer, Thomas, Kristensen, Vessela N, Souček, Pavel, Ojima, Iwao, Kovář, Jan
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - genetics
ATP Binding Cassette Transporter, Subfamily B - genetics
Breast cancer
Cells
Chromosomes
Colorectal cancer
Cyclic AMP
Drug Resistance, Neoplasm - genetics
Estrogens
Female
Fluorescence in situ hybridization
Gene expression
Humans
Hybridization
In Situ Hybridization, Fluorescence
LIM Domain Proteins - genetics
MCF-7 Cells
MicroRNAs
Neoplasms - genetics
Patients
Progesterone
Proteins
Response Elements
Surgery
Taxanes
Taxoids
Transcription Factors - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A limited number of studies are devoted to regulating expression in cancer. Hence, we aimed to unveil the regulation of expression in MCF-7 breast cancer cells (with high expression) and taxane-resistant MCF-7 sublines (manifesting even higher expression). We found that transcription is regulated primarily by the cyclic AMP response element (CRE) in hypomethylated proximal promoters in both taxane-sensitive and taxane-resistant MCF-7 cells. Furthermore, in taxane-resistant MCF-7 sublines, co-amplification with the neighboring gene, as witnessed by fluorescence in situ hybridization (FISH), led to overexpression. Ultimately, we found high mRNA levels in progesterone receptor-positive breast cancer and samples resected from premenopausal women.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes14020296