Antioxidant and Antibacterial Properties of a Functional Sports Beverage Formulation

• Published in: International journal of molecular sciences 2023-02, Vol.24 (4), p.3558
• Main Authors: Kolonas, Alexandros, Vareltzis, Patroklos, Kiroglou, Smaro, Goutzourelas, Nikolaos, Stagos, Dimitrios, Trachana, Varvara, Tsadila, Christina, Mossialos, Dimitris, Mourtakos, Stamatis, Gortzi, Olga
• Format: Article
• Language: English
• Subjects: Acceptance tests; Amino acids; Antibacterial agents; Antioxidants; Beverages; Blood pressure; Caffeine; Catechin; Diabetes; Electrolytes; Endurance; Energy drinks; Epicatechin; Exercise; Food; Functional foods & nutraceuticals; Hypertension; Isotonic beverages; Kaempferol; Mesenchyme; Metabolism; Nutrition; Oxidative stress; Pathogens; Phenolic compounds; Physical fitness; Physiological aspects; Polyphenols; Potassium; Protocatechuic acid; Pseudomonas aeruginosa; Stability analysis; Stem cells; Thiobarbituric acid; Type 2 diabetes
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24043558

Athletes often consume functional beverages in order to improve performance and reduce oxidative stress caused by high-intensity exercise. The present study aimed to evaluate the antioxidant and antibacterial properties of a functional sports beverage formulation. The beverage's antioxidant effects were assessed on human mesenchymal stem cells (MSCs) by determining thiobarbituric acid reactive substances (TBARS; TBARS levels decreased significantly by 52.67% at 2.0 mg/mL), total antioxidant capacity (TAC; TAC levels increased significantly by 80.82% at 2.0 mg/mL) and reduced glutathione (GSH; GSH levels increased significantly by 24.13% at 2.0 mg/mL) levels. Furthermore, the beverage underwent simulated digestion following the INFOGEST protocol to assess its oxidative stability. The analysis of the total phenolic content (TPC) using the Folin-Ciocalteu assay revealed that the beverage contained a TPC of 7.58 ± 0.066 mg GAE/mL, while the phenolics identified by HPLC were catechin (2.149 mg/mL), epicatechin (0.024 mg/mL), protocatechuic acid (0.012 mg/mL), luteolin 7-glucoside (0.001 mg/mL), and kaempferol-3-O-β-rutinoside (0.001 mg/mL). The beverage's TPC was strongly correlated with TAC (R = 896). Moreover, the beverage showcased inhibitory and bacteriostatic effects against and . Lastly, the sensory acceptance test demonstrated that the functional sports beverage was well accepted by the assessors.