Investigating the Ischaemic Phase of Skin NADH Fluorescence Dynamics in Recently Diagnosed Primary Hypertension: A Time Series Analysis

The reduced form of nicotinamide adenine dinucleotide (NADH) is crucial in cellular metabolism. During hypoxia, NADH accumulation results from anaerobic cytoplasmic glycolysis and impaired mitochondrial function. This study aimed to compare the dynamic changes in the 460-nm forearm skin fluorescence...

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Published in:Journal of clinical medicine 2023-02, Vol.12 (4), p.1247
Main Authors: Pawlak-Chomicka, Regina, Chomicki, Wojciech, Krauze, Tomasz, Uruski, Paweł, Guzik, Maria, Piskorski, Jarosław, Tykarski, Andrzej, Guzik, Przemysław
Format: Article
Language:English
Subjects:
Alzheimer's disease
Blood pressure
Cardiovascular disease
Clinical medicine
Development and progression
Diabetes
Diagnosis
Health aspects
Hypertension
Hypoxia
Ischemia
Measurement
Metabolism
Mitochondria
NAD (Coenzyme)
Patients
Physiological aspects
Risk factors
Sample size
Time series
Trends
Values
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Summary:The reduced form of nicotinamide adenine dinucleotide (NADH) is crucial in cellular metabolism. During hypoxia, NADH accumulation results from anaerobic cytoplasmic glycolysis and impaired mitochondrial function. This study aimed to compare the dynamic changes in the 460-nm forearm skin fluorescence, which reflects cellular NADH content, during transient ischaemia between healthy individuals and patients with newly diagnosed, untreated essential hypertension (HA). Sixteen healthy volunteers and sixty-five patients with HA underwent non-invasive measurement of forearm skin NADH content using the Flow Mediated Skin Fluorescence (FMSF) method at rest and during a 100-s transient ischaemia induced by inflation of the brachial cuff. The fluorescent signal was sampled at 25 Hz. All samples were normalised to the end of the ischaemic phase, which is the most stable phase of the whole recording. Slope values of 1 s linear regressions were determined for every 25-sample neighbouring set. The 1-s slopes in the early phase of skin ischaemia, indicating quicker hypoxia-induced NADH accumulation in skin, were significantly higher in patients with HA than in healthy individuals. These findings suggest that some protecting mechanisms postponing the early consequences of early cellular hypoxia and premature NADH accumulation during skin ischaemia are impaired in patients with untreated HA. Further studies are needed to investigate this phenomenon.
ISSN:2077-0383
2077-0383
DOI:10.3390/jcm12041247