Purification and characterization of the soluble methane monooxygenase of the type II methanotrophic bacterium Methylocystis sp. strain WI 14

Methane monooxygenase (MMO) catalyzes the oxidation of methane to methanol as the first step of methane degradation. A soluble NAD(P)H-dependent methane monooxygenase (sMMO) from the type II methanotrophic bacterium WI 14 was purified to homogeneity. Sequencing of the 16S rDNA and comparison with th...

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Bibliographic Details
Published in:Applied and environmental microbiology 1999-09, Vol.65 (9), p.3929-3935
Main Authors: GROSSE, S, LARAMEE, L, WENDLANDT, K.-D, MCDONALD, I. R, MIGUEZ, C. B, KLEBER, H.-P
Format: Article
Language:English
Subjects:
Alphaproteobacteria - enzymology
Alphaproteobacteria - growth & development
Bacteria
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Cloning, Molecular
DNA, Bacterial - genetics
Enzyme Stability
Enzymology and Protein Engineering
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Methane
Methylococcus capsulatus
Methylocystis
Methylosinus trichosporium
Microbiology
Miscellaneous
Mission oriented research
Oxygenases - genetics
Oxygenases - isolation & purification
Oxygenases - metabolism
Phylogeny
Polymerase Chain Reaction - methods
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Solubility
Substrate Specificity
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Summary:Methane monooxygenase (MMO) catalyzes the oxidation of methane to methanol as the first step of methane degradation. A soluble NAD(P)H-dependent methane monooxygenase (sMMO) from the type II methanotrophic bacterium WI 14 was purified to homogeneity. Sequencing of the 16S rDNA and comparison with that of other known methanotrophic bacteria confirmed that strain WI 14 is very close to the genus Methylocystis. The sMMO is expressed only during growth under copper limitation (
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.65.9.3929-3935.1999