Identification and Functional Characterization of Fungal Chalcone Synthase and Chalcone Isomerase

By mining fungal genomic information, a noncanonical iterative type I PKS fused with an N-terminal adenylation–thiolation didomain, which catalyzes the formation of naringenin chalcone, was found. Structural prediction and molecular docking analysis indicated that a C-terminal thioesterase domain wa...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2023-02, Vol.86 (2), p.398-405
Main Authors: Furumura, Sho, Ozaki, Taro, Sugawara, Akihiro, Morishita, Yohei, Tsukada, Kento, Ikuta, Tatsuya, Inoue, Asuka, Asai, Teigo
Format: Article
Language:English
Subjects:
Acyltransferases
Flavonoids - chemistry
Molecular Docking Simulation
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Summary:By mining fungal genomic information, a noncanonical iterative type I PKS fused with an N-terminal adenylation–thiolation didomain, which catalyzes the formation of naringenin chalcone, was found. Structural prediction and molecular docking analysis indicated that a C-terminal thioesterase domain was involved in the Claisen-type cyclization. An enzyme responsible for formation of (2S)-flavanone in the biosynthesis of fungal flavonoids was also identified. Collectively, these findings demonstrate unprecedented fungal biosynthetic machinery leading to plant-like metabolites.
ISSN:0163-3864
1520-6025
DOI:10.1021/acs.jnatprod.2c01027