The Structure and Expression of the Integrated Viral DNA in Mouse Cells Transformed by Simian Virus 40

Mouse cells stably transformed by simian virus 40 (SV40) contain viral DNA covalently integrated into their chromosomal DNA. We have used molecular cloning techniques to isolate and characterize the integrated viral DNA, together with the flanking cellular sequences, from several lines of transforme...

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Bibliographic Details
Published in:Proceedings of the Royal Society of London. Series B, Biological sciences Biological sciences, 1980-11, Vol.210 (1180), p.437-450
Main Authors: Rigby, Peter William Jack, Chia, W., Clayton, Christine E., Lovett, M.
Format: Article
Language:English
Subjects:
3T3 cells
Animals
Antigens, Neoplasm - genetics
Cell lines
Cell Transformation, Viral
DNA
DNA Replication
DNA, Viral - genetics
Gene Expression Regulation
Genes, Viral
Messenger RNA
Mice
Simian virus 40
Simian virus 40 - genetics
Transformed cell line
Tumor cell line
Viral DNA
Viral Proteins - genetics
Viral tumor antigens
Viruses
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Description
Summary:Mouse cells stably transformed by simian virus 40 (SV40) contain viral DNA covalently integrated into their chromosomal DNA. We have used molecular cloning techniques to isolate and characterize the integrated viral DNA, together with the flanking cellular sequences, from several lines of transformed cells. We have identified in SV40-infected mouse cells a novel form of viral DNA, which we believe to be the precursor to the integrated DNA. The mRNAs transcribed from the integrated viral DNA templates have been characterized. Integrating the viral genome into the cellular chromosome can significantly alter its transcription pattern and in several cases the altered mRNAs encode novel forms of viral tumour antigen.
ISSN:0962-8452
0080-4649
0950-1193
1471-2954
2053-9193
DOI:10.1098/rspb.1980.0145