Developing defined substrates for stem cell culture and differentiation

Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons fro...

Full description

Saved in:
Bibliographic Details
Published in:Philosophical transactions of the Royal Society of London. Series B. Biological sciences 2018-07, Vol.373 (1750), p.20170230-20170230
Main Authors: Hagbard, Louise, Cameron, Katherine, August, Paul, Penton, Christopher, Parmar, Malin, Hay, David C., Kallur, Therése
Format: Article
Language:English
Subjects:
Basic Medicine
Cell and Molecular Biology
Cell Culture
Cell Culture Techniques - methods
Cell Differentiation - physiology
Cell fate
Cell Therapy
Cell- och molekylärbiologi
Commercialization
Defined Substrates
Developmental biology
Differentiation
Extracellular matrix
Extracellular Matrix - classification
Extracellular Matrix - physiology
Human Pluripotent Stem Cells
Human Recombinant Laminins
Humans
Laminin
Laminin - physiology
Medical and Health Sciences
Medicin och hälsovetenskap
Medicinska och farmaceutiska grundvetenskaper
Molecular chains
Niches
Pluripotency
Proteins
Reagents
Review
Stem cells
Stem Cells - physiology
Substrates
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons from developmental biology have identified signalling molecules that can guide the differentiation process in vitro, but less attention has been paid to the extracellular matrix used. With the introduction of more biologically relevant and defined matrices, that better mimic specific cell niches, researchers now have powerful resources to fine-tune their in vitro differentiation systems, which may allow the manufacture of therapeutically relevant cell types. In this review article, we revisit the basics of the extracellular matrix, and explore the important role of the cell–matrix interaction. We focus on laminin proteins because they help to maintain pluripotency and drive cell fate specification. This article is part of the theme issue ‘Designer human tissue: coming to a lab near you’.
ISSN:0962-8436
1471-2970
1471-2970
DOI:10.1098/rstb.2017.0230