Bead diffusion assay for discovering antimicrobial cyclic peptidesElectronic supplementary information (ESI) available: Details of library design, synthesis, and screening and synthesis of peptides. See DOI: 10.1039/c0cc04670a

A combinatorial library of 15 536 cyclic decapeptide analogues of tyrocidine A and gramicidin S was prepared on photocleavable TentaGel beads. The beads were photolyzed without solvent, and spread onto an agar plate inoculated with bacterial lawn. Clear zones were formed around beads carrying antimi...

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Bibliographic Details
Main Authors: Fluxà, Viviana S, Maillard, Noélie, Page, Malcolm G. P, Reymond, Jean-Louis
Format: Article
Language:English
Online Access:Get full text
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Summary:A combinatorial library of 15 536 cyclic decapeptide analogues of tyrocidine A and gramicidin S was prepared on photocleavable TentaGel beads. The beads were photolyzed without solvent, and spread onto an agar plate inoculated with bacterial lawn. Clear zones were formed around beads carrying antimicrobially active peptides such as E18 c(kV Orn Lf Thi Y Orn Lq), which inhibited growth of B. subtilis and selected MRSA strains. A bead diffusion assay was developed to identify antimicrobial cyclic peptides in a tyrocidine A and gramicidin S analogue library.
ISSN:1359-7345
1364-548X
DOI:10.1039/c0cc04670a