Voltage controlled nano-injection system for single-cell surgeryElectronic supplementary information (ESI) available. See DOI: 10.1039/c2nr31700a

Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. Herein we describe a single-cell injection platform based on nanopipettes. The system uses scanning microscopy techniques to detect cell surfaces, and voltage pulses...

Full description

Saved in:
Bibliographic Details
Main Authors: Adam Seger, R, Actis, Paolo, Penfold, Catherine, Maalouf, Michelle, Vilozny, Boaz, Pourmand, Nader
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. Herein we describe a single-cell injection platform based on nanopipettes. The system uses scanning microscopy techniques to detect cell surfaces, and voltage pulses to deliver molecules into individual cells. As a proof of concept, we injected adherent mammalian cells with fluorescent dyes. We developed a system that uses scanning microscopy techniques to detect cell surfaces, and voltage pulses to deliver molecules into individual cells.
ISSN:2040-3364
2040-3372
DOI:10.1039/c2nr31700a