A mesofluidic platform integrating on-chip probe ultrasonication for multiple sample pretreatment involving denaturation, reduction, and digestion in protein identification assays by mass spectrometryElectronic supplementary information (ESI) available: Experimental section - chemicals and starting materials and instrumentation methods. See DOI: 10.1039/c3an02178e

The integration of ultrasound (US)-assisted sample processing on-chip in a lab-on-a-valve (LOV) format for automated high-throughput shotgun proteomic assays is herein presented for the first time. The proof of concept of this system was demonstrated with the analysis of three proteins and sera from...

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Main Authors: Nunes-Miranda, J. D, Núñez, Cristina, Santos, Hugo M, Vale, G, Reboiro-Jato, Miguel, Fdez-Riverola, Florentino, Lodeiro, Carlos, Miró, Manuel, Capelo, J. L
Format: Article
Language:English
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Summary:The integration of ultrasound (US)-assisted sample processing on-chip in a lab-on-a-valve (LOV) format for automated high-throughput shotgun proteomic assays is herein presented for the first time. The proof of concept of this system was demonstrated with the analysis of three proteins and sera from patients with lymphoma or myeloma. A novel mesofluidic platform integrating on-chip probe ultrasonication for automated high-throughput shotgun proteomic assays.
ISSN:0003-2654
1364-5528
DOI:10.1039/c3an02178e