Measuring protein reduction potentials using 15N HSQC NMR spectroscopyElectronic supplementary information (ESI) available: Isotopic enrichment notes, protein expression and purification, NMR assignment notes, reduction potential Hill plots for PDI a, ab, ERp18 and DsbA, combined Hill plots of PDI a and ab and oxidised data Hill plots of PDI a and ERp18. See DOI: 10.1039/c3cc38952a

NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple 15 N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 conf...

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Main Authors: Taylor, Samantha L, Crawley-Snowdon, Harriet, Wagstaff, Jane L, Rowe, Michelle L, Shepherd, Mark, Williamson, Richard A, Howard, Mark J
Format: Article
Language:English
Online Access:Get full text
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Summary:NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple 15 N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 confirms this protein as an oxidoreductase of comparable or greater reducing strength than PDI a. Nernst equation measurement of protein reduction potentials using multiple 15 N HSQC NMR resonances: application to the PDI family and DsbA.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc38952a