Molecularly imprinted polymers prepared using protein-conjugated cleavable monomers followed by site-specific post-imprinting introduction of fluorescent reporter moleculesElectronic supplementary information (ESI) available: Synthesis, NMR, MS spectra, measurement procedures, SPR data, and fluorescent spectra. See DOI: 10.1039/c3cc40484f

• Main Authors: Suga, Yusuke, Sunayama, Hirobumi, Ooya, Tooru, Takeuchi, Toshifumi
• Format: Article
• Language: English
• ISSN: 1359-7345; 1364-548X
• DOI: 10.1039/c3cc40484f

Molecularly imprinted polymers were prepared using a protein-conjugated disulfide cleavable monomer. After removing the protein by disulfide reduction, a thiol-reactive fluorophore was introduced into the thiol residue located only inside the imprinted cavity, resulting in specific transduction of the binding events into fluorescence spectral change. Fluorescent reporter molecules were introduced into an imprinted cavity selectively by a site-specific post-imprinting modification.