Exploring the differences between mouse mA142 and human hA142 for Alzheimer's disease related properties and neuronal cytotoxicityElectronic supplementary information (ESI) available: Experimental procedures. See DOI: 10.1039/c3cc40779a

The differences between mouse mA 142 and human hA 142 , explored using CD and fluorescence spectroscopy, transmission electron microscopy, ROS fluorescent assay, and neuronal cell viability, revealed that mA 142 as a three-site mutant (R5G, Y10F and H13R) of hA 142 altered the metal (copper and zinc...

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Main Authors: Lv, Xiaoping, Li, Wei, Luo, Ying, Wang, Dandan, Zhu, Cuiqing, Huang, Zhong-Xian, Tan, Xiangshi
Format: Article
Language:English
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Summary:The differences between mouse mA 142 and human hA 142 , explored using CD and fluorescence spectroscopy, transmission electron microscopy, ROS fluorescent assay, and neuronal cell viability, revealed that mA 142 as a three-site mutant (R5G, Y10F and H13R) of hA 142 altered the metal (copper and zinc) binding sites, reduced the proneness to form -sheet structures and aggregated fibrils, alleviated the generation of ROS, and decreased the cytotoxicity, in contrast to hA 142 . The differences between mA 142 and hA 142 for Alzheimer's disease related properties and neurotoxicity were explored and the mechanism was investigated.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc40779a