The first latent green fluorophores for the detection of azoreductase activity in bacterial culturesElectronic supplementary information (ESI) available: Experimental procedures and characterisation/spectral data for all compounds. See DOI: 10.1039/c3cc44798g

An efficient synthesis of original bio-reductive probes suitable for the detection of azoreductases from the fluorescent rhodamine 110 dye is presented. A "turn-on" green fluorescence response upon reduction of the two diazo bonds of these latent fluorophores was observed both in vitro and...

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Main Authors: Chevalier, Arnaud, Mercier, Claire, Saurel, Laura, Orenga, Sylvain, Renard, Pierre-Yves, Romieu, Anthony
Format: Article
Language:English
Online Access:Get full text
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Summary:An efficient synthesis of original bio-reductive probes suitable for the detection of azoreductases from the fluorescent rhodamine 110 dye is presented. A "turn-on" green fluorescence response upon reduction of the two diazo bonds of these latent fluorophores was observed both in vitro and in the context of bacterial cultures. Bis azo-coupling reaction between the fluorescent rhodamine 110 dye and N , N -dialkyl substituted anilines has led to the first green-emitting pro-fluorophores suitable for azoreductase sensing in bacterial cultures.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc44798g