Functional isoDNA aptamers: modified thrombin binding aptamers with a 2′-5′-linked sugar-phosphate backbone (isoTBA)Electronic supplementary information (ESI) available: Experimental conditions and spectral data figures. See DOI: 10.1039/c3cc47569g

• Main Authors: Gunjal, Anita D, Fernandes, Moneesha, Erande, Namrata, Rajamohanan, P. R, Kumar, Vaijayanti A
• Format: Article
• Language: English
• ISSN: 1359-7345; 1364-548X
• DOI: 10.1039/c3cc47569g

The regioisomeric 3′-deoxy-2′-5′-linked thrombin binding DNA aptamers (isoTBAs) were chemically synthesized and their ability to form unimolecular anti-parallel G-quadruplexes in the presence of K + ions was evaluated. These modified sequences retain the function of the native thrombin binding aptamer (TBA), exhibit better stability against exonuclease and are capable of slowing down the process of blood clotting. An isomeric 2′-5′-DNA aptamer (isoTBA) of the SELEX-derived thrombin binding DNA aptamer (TBA) forms a quadruplex fold with a chaperone-like activity of the large protein thrombin, or in the presence of cations such as K + /Na + . Its activity in the process of blood clot formation was also confirmed.