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Suppression of ZIP8 expression is a common feature of cadmium-resistant and manganese-resistant RBL-2H3 cellsElectronic supplementary information (ESI) available. See DOI: 10.1039/c3mt00003f

Rat basophilic leukemia RBL-2H3 cells show markedly high sensitivity to both CdCl 2 and MnCl 2 compared with other rat cell lines, due to efficient accumulation of cadmium and manganese. To clarify the roles of metal transporters in hyperaccumulation of cadmium and manganese in RBL-2H3 cells, Cd-res...

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Bibliographic Details
Main Authors: Fujishiro, Hitomi, Ohashi, Toshinao, Takuma, Miki, Himeno, Seiichiro
Format: Article
Language:English
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Summary:Rat basophilic leukemia RBL-2H3 cells show markedly high sensitivity to both CdCl 2 and MnCl 2 compared with other rat cell lines, due to efficient accumulation of cadmium and manganese. To clarify the roles of metal transporters in hyperaccumulation of cadmium and manganese in RBL-2H3 cells, Cd-resistant and Mn-resistant cells were developed from RBL-2H3 cells by continuous exposure to CdCl 2 and MnCl 2 , respectively. The established Cd-resistant (RBL-Cdr) and Mn-resistant (RBL-Mnr) cells exhibited about 20 times higher LC 50 values of CdCl 2 and MnCl 2 , respectively, than parental RBL-2H3 cells, and showed cross-resistance to each metal. The resistance to cadmium and manganese was primarily conferred by a marked decrease in the uptake of both metals. RBL-Cdr cells also showed cross-resistance to HgCl 2 and AgNO 3 probably due to enhanced expression of metallothionein. Among the possible transporters involved in the uptake of Cd 2+ and Mn 2+ , the expression of ZIP8 (Zrt-, Irt-related protein 8), encoded by Slc39a8 , showed a marked suppression in both RBL-Cdr and RBL-Mnr cells. These results suggest that ZIP8 plays a pivotal role in the transport and toxicity of Cd 2+ and Mn 2+ in RBL-2H3 cells. Cd-resistant and Mn-resistant cells established from RBL-2H3 cells showed the suppression of ZIP8 which is involved in the transport of both Cd and Mn.
ISSN:1756-5901
1756-591X
DOI:10.1039/c3mt00003f