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A cascading reaction sequence involving ligand-directed azaelectrocyclization and autooxidation-induced fluorescence recovery enables visualization of target proteins on the surfaces of live cellsElectronic supplementary information (ESI) available: Experimental details, characterization data and selected copies of NMR spectra. See DOI: 10.1039/c3ob42267d

A general probe designed to induce a cascading sequence of reactions on a target protein was efficiently synthesized. The cascading reaction sequence involved (i) ligand-directed azaelectrocyclization with lysine and (ii) the autooxidation-induced release of a fluorescence quencher from the labeled...

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Main Authors: Tanaka, Katsunori, Kitadani, Masataka, Tsutsui, Ayumi, Pradipta, Ambara R, Imamaki, Rie, Kitazume, Shinobu, Taniguchi, Naoyuki, Fukase, Koichi
Format: Article
Language:English
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Summary:A general probe designed to induce a cascading sequence of reactions on a target protein was efficiently synthesized. The cascading reaction sequence involved (i) ligand-directed azaelectrocyclization with lysine and (ii) the autooxidation-induced release of a fluorescence quencher from the labeled protein. The probe was linked to a cyclic RGDyK peptide to enable the selective visualization of integrin α V β 3 on the surfaces of live cells. A general probe designed to induce a cascading sequence of reactions on a target protein was efficiently synthesized.
ISSN:1477-0520
1477-0539
DOI:10.1039/c3ob42267d