Amperometric biosensor precision improvement: application to organophosphorus pesticide determination

An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized. The enzyme organophosphorus hydrolase was employed as a specific agent for electrode surface modification, and the pulsed amperometric detection for 66.6 ms was applied for the...

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Bibliographic Details
Published in:Analytical methods 2014-01, Vol.6 (2), p.8232-8238
Main Authors: Stoytcheva, M, Zlatev, R, Gochev, V, Velkova, Z, Montero, G
Format: Article
Language:English
Subjects:
Cleaning
Electrical measurements
Electrochemical cleaning
Electrodes
Enzymes
Hydrolysis
Mathematical analysis
Pesticides
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Summary:An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized. The enzyme organophosphorus hydrolase was employed as a specific agent for electrode surface modification, and the pulsed amperometric detection for 66.6 ms was applied for the hydrolysis product detection. Pulsed electrochemical cleaning, i.e. oxidation at a very positive potential (+1.4 V vs. Ag, AgCl) was applied every 233 ms for 166.6 ms for elimination of the fouling effect, which degrades the determination precision. By this way a RSD of 0.41% at 40 μmol L −1 p -nitrophenol determination was achieved after 10 determinations, instead of 43.93% without the pulsed cleaning application. An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized.
ISSN:1759-9660
1759-9679
DOI:10.1039/c4ay01792g