Amperometric biosensor precision improvement: application to organophosphorus pesticide determination

An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized. The enzyme organophosphorus hydrolase was employed as a specific agent for electrode surface modification, and the pulsed amperometric detection for 66.6 ms was applied for the...

Full description

Saved in:
Bibliographic Details
Published in:Analytical methods 2014-01, Vol.6 (2), p.8232-8238
Main Authors: Stoytcheva, M, Zlatev, R, Gochev, V, Velkova, Z, Montero, G
Format: Article
Language:English
Subjects:
Cleaning
Electrical measurements
Electrochemical cleaning
Electrodes
Enzymes
Hydrolysis
Mathematical analysis
Pesticides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c349t-1d48d5fd9ed3aefa502e9acbdd5fd356c64857520db9da530ea41d99f967b9483
cites cdi_FETCH-LOGICAL-c349t-1d48d5fd9ed3aefa502e9acbdd5fd356c64857520db9da530ea41d99f967b9483
container_end_page 8238
container_issue 2
container_start_page 8232
container_title Analytical methods
container_volume 6
creator Stoytcheva, M
Zlatev, R
Gochev, V
Velkova, Z
Montero, G
description An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized. The enzyme organophosphorus hydrolase was employed as a specific agent for electrode surface modification, and the pulsed amperometric detection for 66.6 ms was applied for the hydrolysis product detection. Pulsed electrochemical cleaning, i.e. oxidation at a very positive potential (+1.4 V vs. Ag, AgCl) was applied every 233 ms for 166.6 ms for elimination of the fouling effect, which degrades the determination precision. By this way a RSD of 0.41% at 40 μmol L −1 p -nitrophenol determination was achieved after 10 determinations, instead of 43.93% without the pulsed cleaning application. An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized.
doi_str_mv 10.1039/c4ay01792g
format article
fullrecord <record><control><sourceid>proquest_rsc_p</sourceid><recordid>TN_cdi_rsc_primary_c4ay01792g</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1685774061</sourcerecordid><originalsourceid>FETCH-LOGICAL-c349t-1d48d5fd9ed3aefa502e9acbdd5fd356c64857520db9da530ea41d99f967b9483</originalsourceid><addsrcrecordid>eNp9kEFLAzEQhYMoWKsX70K8ibCaNNls460UrULBix48hWwyWyO7m5hshf5701b05mGYYd43w8xD6JySG0qYvDVcbwit5GR1gEa0KmUhRSUPf2tBjtFJSh-ECMkEHSGYdQGi72CIzuDa-QR98hGHCMYl53vsuhD9F3TQD3dYh9A6o4etMHjs40r3Prz7lCOuEw6QBmecBWxhgNi5fseeoqNGtwnOfvIYvT7cv8wfi-Xz4mk-WxaGcTkU1PKpLRsrwTINjS7JBKQ2td02WSmM4NOyKifE1tLqkhHQnFopm_xkLfmUjdHVfm8--XOdb1GdSwbaVvfg10lRkecrTgTN6PUeNdGnFKFRIbpOx42iRG29VHM-e9t5ucjwxR6Oyfxyf15n_fI_XQXbsG8w74CH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1685774061</pqid></control><display><type>article</type><title>Amperometric biosensor precision improvement: application to organophosphorus pesticide determination</title><source>Royal Society of Chemistry</source><creator>Stoytcheva, M ; Zlatev, R ; Gochev, V ; Velkova, Z ; Montero, G</creator><creatorcontrib>Stoytcheva, M ; Zlatev, R ; Gochev, V ; Velkova, Z ; Montero, G</creatorcontrib><description>An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized. The enzyme organophosphorus hydrolase was employed as a specific agent for electrode surface modification, and the pulsed amperometric detection for 66.6 ms was applied for the hydrolysis product detection. Pulsed electrochemical cleaning, i.e. oxidation at a very positive potential (+1.4 V vs. Ag, AgCl) was applied every 233 ms for 166.6 ms for elimination of the fouling effect, which degrades the determination precision. By this way a RSD of 0.41% at 40 μmol L −1 p -nitrophenol determination was achieved after 10 determinations, instead of 43.93% without the pulsed cleaning application. An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized.</description><identifier>ISSN: 1759-9660</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/c4ay01792g</identifier><language>eng</language><subject>Cleaning ; Electrical measurements ; Electrochemical cleaning ; Electrodes ; Enzymes ; Hydrolysis ; Mathematical analysis ; Pesticides</subject><ispartof>Analytical methods, 2014-01, Vol.6 (2), p.8232-8238</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-1d48d5fd9ed3aefa502e9acbdd5fd356c64857520db9da530ea41d99f967b9483</citedby><cites>FETCH-LOGICAL-c349t-1d48d5fd9ed3aefa502e9acbdd5fd356c64857520db9da530ea41d99f967b9483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Stoytcheva, M</creatorcontrib><creatorcontrib>Zlatev, R</creatorcontrib><creatorcontrib>Gochev, V</creatorcontrib><creatorcontrib>Velkova, Z</creatorcontrib><creatorcontrib>Montero, G</creatorcontrib><title>Amperometric biosensor precision improvement: application to organophosphorus pesticide determination</title><title>Analytical methods</title><description>An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized. The enzyme organophosphorus hydrolase was employed as a specific agent for electrode surface modification, and the pulsed amperometric detection for 66.6 ms was applied for the hydrolysis product detection. Pulsed electrochemical cleaning, i.e. oxidation at a very positive potential (+1.4 V vs. Ag, AgCl) was applied every 233 ms for 166.6 ms for elimination of the fouling effect, which degrades the determination precision. By this way a RSD of 0.41% at 40 μmol L −1 p -nitrophenol determination was achieved after 10 determinations, instead of 43.93% without the pulsed cleaning application. An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized.</description><subject>Cleaning</subject><subject>Electrical measurements</subject><subject>Electrochemical cleaning</subject><subject>Electrodes</subject><subject>Enzymes</subject><subject>Hydrolysis</subject><subject>Mathematical analysis</subject><subject>Pesticides</subject><issn>1759-9660</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kEFLAzEQhYMoWKsX70K8ibCaNNls460UrULBix48hWwyWyO7m5hshf5701b05mGYYd43w8xD6JySG0qYvDVcbwit5GR1gEa0KmUhRSUPf2tBjtFJSh-ECMkEHSGYdQGi72CIzuDa-QR98hGHCMYl53vsuhD9F3TQD3dYh9A6o4etMHjs40r3Prz7lCOuEw6QBmecBWxhgNi5fseeoqNGtwnOfvIYvT7cv8wfi-Xz4mk-WxaGcTkU1PKpLRsrwTINjS7JBKQ2td02WSmM4NOyKifE1tLqkhHQnFopm_xkLfmUjdHVfm8--XOdb1GdSwbaVvfg10lRkecrTgTN6PUeNdGnFKFRIbpOx42iRG29VHM-e9t5ucjwxR6Oyfxyf15n_fI_XQXbsG8w74CH</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Stoytcheva, M</creator><creator>Zlatev, R</creator><creator>Gochev, V</creator><creator>Velkova, Z</creator><creator>Montero, G</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20140101</creationdate><title>Amperometric biosensor precision improvement: application to organophosphorus pesticide determination</title><author>Stoytcheva, M ; Zlatev, R ; Gochev, V ; Velkova, Z ; Montero, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-1d48d5fd9ed3aefa502e9acbdd5fd356c64857520db9da530ea41d99f967b9483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Cleaning</topic><topic>Electrical measurements</topic><topic>Electrochemical cleaning</topic><topic>Electrodes</topic><topic>Enzymes</topic><topic>Hydrolysis</topic><topic>Mathematical analysis</topic><topic>Pesticides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stoytcheva, M</creatorcontrib><creatorcontrib>Zlatev, R</creatorcontrib><creatorcontrib>Gochev, V</creatorcontrib><creatorcontrib>Velkova, Z</creatorcontrib><creatorcontrib>Montero, G</creatorcontrib><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stoytcheva, M</au><au>Zlatev, R</au><au>Gochev, V</au><au>Velkova, Z</au><au>Montero, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amperometric biosensor precision improvement: application to organophosphorus pesticide determination</atitle><jtitle>Analytical methods</jtitle><date>2014-01-01</date><risdate>2014</risdate><volume>6</volume><issue>2</issue><spage>8232</spage><epage>8238</epage><pages>8232-8238</pages><issn>1759-9660</issn><eissn>1759-9679</eissn><abstract>An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized. The enzyme organophosphorus hydrolase was employed as a specific agent for electrode surface modification, and the pulsed amperometric detection for 66.6 ms was applied for the hydrolysis product detection. Pulsed electrochemical cleaning, i.e. oxidation at a very positive potential (+1.4 V vs. Ag, AgCl) was applied every 233 ms for 166.6 ms for elimination of the fouling effect, which degrades the determination precision. By this way a RSD of 0.41% at 40 μmol L −1 p -nitrophenol determination was achieved after 10 determinations, instead of 43.93% without the pulsed cleaning application. An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized.</abstract><doi>10.1039/c4ay01792g</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1759-9660
ispartof Analytical methods, 2014-01, Vol.6 (2), p.8232-8238
issn 1759-9660
1759-9679
language eng
recordid cdi_rsc_primary_c4ay01792g
source Royal Society of Chemistry
subjects Cleaning
Electrical measurements
Electrochemical cleaning
Electrodes
Enzymes
Hydrolysis
Mathematical analysis
Pesticides
title Amperometric biosensor precision improvement: application to organophosphorus pesticide determination
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-24T01%3A53%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_rsc_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Amperometric%20biosensor%20precision%20improvement:%20application%20to%20organophosphorus%20pesticide%20determination&rft.jtitle=Analytical%20methods&rft.au=Stoytcheva,%20M&rft.date=2014-01-01&rft.volume=6&rft.issue=2&rft.spage=8232&rft.epage=8238&rft.pages=8232-8238&rft.issn=1759-9660&rft.eissn=1759-9679&rft_id=info:doi/10.1039/c4ay01792g&rft_dat=%3Cproquest_rsc_p%3E1685774061%3C/proquest_rsc_p%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c349t-1d48d5fd9ed3aefa502e9acbdd5fd356c64857520db9da530ea41d99f967b9483%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1685774061&rft_id=info:pmid/&rfr_iscdi=true