Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactionsElectronic supplementary information (ESI) available: Experimental details. See DOI: 10.1039/c4ay02693d

A highly sensitive spectrophotometric method for ethyl carbamate (EC) determination was established through glutamate dehydrogenase/urethanase cascade reactions and the corresponding change in NADH concentration. The absorbance at 340 nm is linearly related to the EC concentration within the range o...

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Main Authors: Lu, Xiaoxia, Zhou, Nandi, Tian, Yaping
Format: Article
Language:English
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Summary:A highly sensitive spectrophotometric method for ethyl carbamate (EC) determination was established through glutamate dehydrogenase/urethanase cascade reactions and the corresponding change in NADH concentration. The absorbance at 340 nm is linearly related to the EC concentration within the range of 0.3-50 μM, with a low detection limit of 0.00928 μM. The assay was further applied to analyse EC in mimic Chinese rice wine samples. Spectrophotometric determination of ethyl carbamate was established based on urethanase/glutamate dehydrogenase cascade reactions and the corresponding change in NADH concentration.
ISSN:1759-9660
1759-9679
DOI:10.1039/c4ay02693d