Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imagingDedicated to Professor Seiji Shinkai on the occasion of his 70th birthday.Electronic supplementary information (ESI) available: Fig. S1-S12 and experimental details. See DOI: 10.1039/c4cc01753f

We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily convert...

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Main Authors: Nakamura, Akinobu, Takigawa, Kazumasa, Kurishita, Yasutaka, Kuwata, Keiko, Ishida, Manabu, Shimoda, Yasushi, Hamachi, Itaru, Tsukiji, Shinya
Format: Article
Language:English
Online Access:Get full text
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Summary:We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes. Attachment of the Hoechst tag to fluorescent molecules allows the creation of (turn-on) fluorescent nucleus-imaging probes.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc01753f