In situ labelling chemistry of respiratory syncytial viruses by employing the biotinylated host-cell membrane protein for tracking the early stage of virus entryElectronic supplementary information (ESI) available: Experimental details and additional figures (Fig. S1-S8). See DOI: 10.1039/c4cc06264g

An in situ labelling strategy was proposed to produce quantum dot-labelled respiratory syncytial viruses (RSVs) by incorporating the biotinylated membrane protein of the host cells into mature virions, followed by conjugation with streptavidin modified quantum dots (SA-QDs), which has the advantages...

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Main Authors: Zheng, Lin Ling, Yang, Xiao Xi, Liu, Yue, Wan, Xiao Yan, Wu, Wen Bi, Wang, Ting Ting, Wang, Qiang, Zhen, Shu Jun, Huang, Cheng Zhi
Format: Article
Language:English
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Summary:An in situ labelling strategy was proposed to produce quantum dot-labelled respiratory syncytial viruses (RSVs) by incorporating the biotinylated membrane protein of the host cells into mature virions, followed by conjugation with streptavidin modified quantum dots (SA-QDs), which has the advantages such as convenience, efficiency and minor influence on viral infectivity and thus could be successfully applied to track the early stage of virus entry. An in situ strategy for producing quantum dot-labelled respiratory syncytial viruses by incorporating the biotinylated membrane protein of the host cells into mature virions is reported.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc06264g