Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acidsElectronic supplementary information (ESI) available: Experimental methods and supporting figures. See DOI: 10.1039/c4cc07778d

Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-ty...

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Bibliographic Details
Main Authors: Piscotta, Frank J, Tharp, Jeffery M, Liu, Wenshe R, Link, A. James
Format: Article
Language:English
Online Access:Get full text
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Summary:Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides. Non-canonical amino acids have been introduced to the lasso peptide microcin J25.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc07778d