Compact multi-enzyme pathways in P. pastorisElectronic supplementary information (ESI) available: Western blot analysis of the preliminary experiments and experiments including a ubiquitin tag to demonstrate the feasibility to create authentic N-termini as well as experimental details and sequence information. See DOI: 10.1039/c4cc08502g

We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the...

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Main Authors: Geier, Martina, Fauland, Pia, Vogl, Thomas, Glieder, Anton
Format: Article
Language:English
Online Access:Get full text
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Summary:We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways. 2A peptides enabling the coordinate expression of nine genes in P. pastoris represent a valuable tool for pathway construction and engineering.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc08502g