Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiationElectronic supplementary information (ESI) available: Experimental details, characterization (FT-IR, XPS, XRD and TGA analysis), alkaline phosphatase (ALP) activity assay, and in vitro tests (osteoblast differentiation, biocompatibility tests, ALP activity assay). See DOI: 10.1039/c4cc09620g

Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity. AuFHAp showed NIR fluorescence due to the hydrolysis of its phosphate groups by ALP. In addition, gold nanoparticles help reduce the nonspecific sign...

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Main Authors: Lim, Eun-Kyung, Keem, Joo Oak, Yun, Hui-suk, Jung, Jinyoung, Chung, Bong Hyun
Format: Article
Language:English
Online Access:Get full text
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Summary:Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity. AuFHAp showed NIR fluorescence due to the hydrolysis of its phosphate groups by ALP. In addition, gold nanoparticles help reduce the nonspecific signal by absorbing nonspecific fluorescence. Through in vitro tests, we confirmed that the AuFHAp probe was capable of detecting ALP levels related to osteoblast activity in living cells with high fluorescence intensity. Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc09620g