Isothermal strand displacement amplification (iSDA): a rapid and sensitive method of nucleic acid amplification for point-of-care diagnosisElectronic supplementary information (ESI) available. See DOI: 10.1039/c5an01632k

We present a method of rapid isothermal amplification of DNA without initial heat denaturation of the template, and methods and probes for (a) real-time fluorescence detection and (b) lateral flow detection of amplicons. Isothermal strand displacement amplification (iSDA) can achieve >10 9 -fold...

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Main Authors: Toley, Bhushan J, Covelli, Isabela, Belousov, Yevgeniy, Ramachandran, Sujatha, Kline, Enos, Scarr, Noah, Vermeulen, Nic, Mahoney, Walt, Lutz, Barry R, Yager, Paul
Format: Article
Language:English
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Summary:We present a method of rapid isothermal amplification of DNA without initial heat denaturation of the template, and methods and probes for (a) real-time fluorescence detection and (b) lateral flow detection of amplicons. Isothermal strand displacement amplification (iSDA) can achieve >10 9 -fold amplification of the target sequence in
ISSN:0003-2654
1364-5528
DOI:10.1039/c5an01632k