Ferricyanide-backfilled cylindrical carbon fiber microelectrodes for in vivo analysis with high stability and low polarized potential

The development of stable and reproducible methods for in vivo electrochemical monitoring of neurochemicals is of great physiological importance. In this study, we demonstrate ferricyanide-filled cylindrical carbon fiber microelectrodes (CFEs) of high stability and low polarized potential for in viv...

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Bibliographic Details
Published in:Analyst (London) 2015-11, Vol.14 (21), p.7154-7159
Main Authors: Zhong, Peipei, Yu, Ping, Wang, Kai, Hao, Jie, Fei, Junjie, Mao, Lanqun
Format: Article
Language:English
Subjects:
Animals
Brain Chemistry
Carbon - chemistry
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Ferricyanides - chemistry
Male
Microelectrodes
Oxidation-Reduction
Rats
Rats, Sprague-Dawley
Reproducibility of Results
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Summary:The development of stable and reproducible methods for in vivo electrochemical monitoring of neurochemicals is of great physiological importance. In this study, we demonstrate ferricyanide-filled cylindrical carbon fiber microelectrodes (CFEs) of high stability and low polarized potential for in vivo electrochemical analysis. We first studied the voltammetric behavior of cylindrical CFEs by using a model system consisting of two separated cells each containing potassium ferricyanide (K 3 Fe(CN) 6 ) or potassium ferrocyanide (K 4 Fe(CN) 6 ). We observed that E 1/2 values of the system were dependent on the ratio of the lengths of the cylindrical CFEs and of the concentrations of the redox species on both poles. Based on this property, we prepared the ferricyanide-backfilled cylindrical CFEs, and found that this kind of electrode exhibits a more stable current response and a lower polarized potential than the CFEs backfilled with KCl or Ru(NH 3 ) 6 Cl 3 . Animal experiments with the ferricyanide-backfilled cylindrical CFEs demonstrate that this kind of electrode could be used for in vivo monitoring of neurochemical release with a high stability under some physiological conditions. A ferricyanide-backfilled cylindrical carbon fiber microelectrode of high stability and low polarized potential was fabricated and used for in vivo analysis.
ISSN:0003-2654
1364-5528
DOI:10.1039/c5an01650a