Loading…

A digital image-based method employing a spot-test for quantification of ethanol in drinksElectronic supplementary information (ESI) available. See DOI: 10.1039/c5ay00529a

A low-cost analytical method for quantification of ethanol in drinks based on the combination of a colorimetric spot-test and a digital image-based (DIB) method is proposed. The digital images from spot-test reactions were captured using a digital camera in a portable plastic chamber designed with i...

Full description

Saved in:
Bibliographic Details
Main Authors: Benedetti, Luzia Pires dos Santos, dos Santos, Vagner Bezerra, Silva, Tiago Almeida, Filho, Edemar Benedetti, Martins, Valdomiro Lacerda, Fatibello-Filho, Orlando
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A low-cost analytical method for quantification of ethanol in drinks based on the combination of a colorimetric spot-test and a digital image-based (DIB) method is proposed. The digital images from spot-test reactions were captured using a digital camera in a portable plastic chamber designed with internal lighting control. The images were decomposed by a RGB approach using freely available software. The R channel showed the best linearity, with two linear ethanol concentration ranges: from 1.0% to 20.0% v/v ( r = 0.999) and from 25.0% to 50.0% v/v ( r = 0.980), with limits of detection and quantification of 0.25% and 0.85% v/v, respectively, for the first analytical curve. The developed method was applied to quantification of ethanol in alcoholic drink samples with results in close agreement with those obtained using a spectrophotometric method at a confidence level of 95%, and with low waste generation (835 μL/spot-test). Thus, we believe that the DIB method can be useful with regard to environmental and social impacts, once the method has a low waste generation and uses an easily available instrumentation with potential for in situ determination during the alcoholic beverage production and its quality control. The digital image analysis together with spot-test based on a colorimetric assay produces a fast, low waste, screening and in situ analysis.
ISSN:1759-9660
1759-9679
DOI:10.1039/c5ay00529a