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A digital image-based method employing a spot-test for quantification of ethanol in drinksElectronic supplementary information (ESI) available. See DOI: 10.1039/c5ay00529a
A low-cost analytical method for quantification of ethanol in drinks based on the combination of a colorimetric spot-test and a digital image-based (DIB) method is proposed. The digital images from spot-test reactions were captured using a digital camera in a portable plastic chamber designed with i...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | A low-cost analytical method for quantification of ethanol in drinks based on the combination of a colorimetric spot-test and a digital image-based (DIB) method is proposed. The digital images from spot-test reactions were captured using a digital camera in a portable plastic chamber designed with internal lighting control. The images were decomposed by a RGB approach using freely available software. The R channel showed the best linearity, with two linear ethanol concentration ranges: from 1.0% to 20.0% v/v (
r
= 0.999) and from 25.0% to 50.0% v/v (
r
= 0.980), with limits of detection and quantification of 0.25% and 0.85% v/v, respectively, for the first analytical curve. The developed method was applied to quantification of ethanol in alcoholic drink samples with results in close agreement with those obtained using a spectrophotometric method at a confidence level of 95%, and with low waste generation (835 μL/spot-test). Thus, we believe that the DIB method can be useful with regard to environmental and social impacts, once the method has a low waste generation and uses an easily available instrumentation with potential for
in situ
determination during the alcoholic beverage production and its quality control.
The digital image analysis together with spot-test based on a colorimetric assay produces a fast, low waste, screening and
in situ
analysis. |
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ISSN: | 1759-9660 1759-9679 |
DOI: | 10.1039/c5ay00529a |