A novel chemiluminescence enhanced method for determination of putrescine in shrimp based on the luminol-[Ag(HIO)] reaction

• Published in: Analytical methods 2016-02, Vol.8 (5), p.1151-1156
• Main Authors: Chen, Peiyun, Sun, Hanwen, Wang, Xianyou, Pang, Yanping, Li, Bin
• Format: Article
• Language: English
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/c5ay02596f

A novel chemiluminescence (CL) method to determine putrescine was proposed based on the enhanced effect of putrescine on CL emission of the [Ag(HIO 6 ) 2 ] 5− -luminol system. The enhanced degree of CL emission was proportional to the putrescine concentration. Under optimal conditions, there was a linear relationship between the increase in CL intensity and the putrescine concentration over a range of 4.0 × 10 −8 to 4 × 10 −6 g mL with a detection limit (3 σ ) of 1.76 × 10 −8 g mL −1 . The relative standard deviation (RSD) was 3.2% for 11 replicate determinations of 1.0 × 10 −6 g mL −1 putrescine. The proposed CL reaction systems had potential capability for residue analysis of studied analytes in food samples. A possible mechanism of CL emission and enhanced effect was also suggested. A novel chemiluminescence enhanced method for the determination of putrescine in shrimp based on the luminol-[Ag(HIO 6 ) 2 ] 5− reaction.