The determination of DNA methyltransferase activity by quenching of tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence with ferroceneElectronic supplementary information (ESI) available. See DOI: 10.1039/c5cc02817e

An electrogenerated chemiluminescence (ECL) biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2′-bipyridine)ruthenium ECL by ferrocene, and it is demonstrated that the ECL biosensing method measures DNA adenine methylation methyltransfe...

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Main Authors: Luo, Xiaoe, Li, Yan, Zheng, Jianbin, Qi, Honglan, Liang, Zhenxing, Ning, Xiaohui
Format: Article
Language:English
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Summary:An electrogenerated chemiluminescence (ECL) biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2′-bipyridine)ruthenium ECL by ferrocene, and it is demonstrated that the ECL biosensing method measures DNA adenine methylation methyltransferase over a dynamic concentration range (0.1 U mL −1 -100 U mL −1 ) with an extremely low detection limit of 0.03 U mL −1 , using gold nanoparticles and a quenching ECL signal produced by a chemical quencher such as ferrocene. An electrogenerated chemiluminescence biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2′-bipyridine)ruthenium ECL by ferrocene.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc02817e