Purple carrot anthocyanins suppress lipopolysaccharide-induced inflammation in the co-culture of intestinal Caco-2 and macrophage RAW264.7 cells

This study was designed to evaluate the anti-inflammatory effects of purple carrot anthocyanins (PCA) with respect to gut inflammation, simulated in a co-culture system consisting of intestinal epithelial Caco-2 cells and RAW264.7 macrophages. The obtained results indicated that PCA extract down-reg...

Full description

Saved in:
Bibliographic Details
Published in:Food & function 2016-01, Vol.7 (1), p.557-564
Main Authors: Olejnik, Anna, Kowalska, Katarzyna, Kido, Marcin, Czapski, Janusz, Rychlik, Joanna, Olkowicz, Mariola, Dembczy ski, Rados aw
Format: Article
Language:English
Subjects:
Animals
Anthocyanins - chemistry
Anthocyanins - pharmacology
Biological Transport
Caco-2 Cells
Cell Line
Coculture Techniques
Daucus
Daucus carota - chemistry
Humans
Inflammation - chemically induced
Inflammation - drug therapy
Lipopolysaccharides - toxicity
Macrophages - drug effects
Mice
Permeability
Plant Extracts - chemistry
RAW 264.7 Cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study was designed to evaluate the anti-inflammatory effects of purple carrot anthocyanins (PCA) with respect to gut inflammation, simulated in a co-culture system consisting of intestinal epithelial Caco-2 cells and RAW264.7 macrophages. The obtained results indicated that PCA extract down-regulates the mRNA expression of proinflammatory interleukins Il-1β (↓91%) and Il-6 (↓69%) as well as inflammatory mediators, such as cyclooxygenase-2 (Cox-2) and inducible nitric oxide synthase (iNos), in lipopolysaccharide-activated RAW264.7 cells. The decrease in the generation of prostaglandin E 2 (↓48%) and nitric oxide (↓26%) was observed as a result of the inhibition of Cox-2 (↓25%) and iNos (↓12%) mRNA expressions, respectively. Moreover, the PCA reduced mRNA expression (↓40%) and production (↓17%) of IL-8 in intestinal cells. The anti-inflammatory effect of PCA was contributed to the protection of the intestinal barrier, which was disrupted upon the stimulation of macrophages. These findings may provide preliminary justification for the use of PCA in further studies focused on the prevention and therapy supporting the conventional treatment of inflammatory bowel diseases. Purple carrot anthocyanins suppress inflammatory pathways by down-regulation of the expression of proinflammatory genes and mediators.
ISSN:2042-6496
2042-650X
DOI:10.1039/c5fo00890e