A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein productionElectronic supplementary information (ESI) available. See DOI: 10.1039/c5lc00443h

We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also al...

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Bibliographic Details
Main Authors: Mozdzierz, Nicholas J, Love, Kerry R, Lee, Kevin S, Lee, Harry L. T, Shah, Kartik A, Ram, Rajeev J, Love, J. Christopher
Format: Article
Language:English
Online Access:Get full text
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Summary:We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing. This work presents an integrated microfluidic perfusion bioreactor for the continuous expression of heterologous proteins from suspended microbial cell cultures.
ISSN:1473-0197
1473-0189
DOI:10.1039/c5lc00443h