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Versatile multiple protein nanopatterning within a microfluidic channel for cell recruitment studiesElectronic supplementary information (ESI) available: Colloidal particle deposition schematic, intensity profile of fluorescence distributions across the channel, the reversible nature of HIS-tag binding, and cell tracks after removal of P-selectin are given as supplementary materials. Also, a video showing cells rolling on the ICAM1/P-selectin nanopattern is available. See DOI: 10.1039/c5lc00916b

A novel approach combining self-assembly-based colloidal lithography and polydimethylsiloxane (PDMS) micromolding to generate complex protein nanopatterns for studying the mechanisms of leukocyte extravasation within microchannels is presented. Nanostructured surfaces sealed onto PDMS-molded microch...

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Bibliographic Details
Main Authors: Andersen, A. S, Zheng, W. F, Sutherland, D. S, Jiang, X. Y
Format: Article
Language:English
Online Access:Get full text
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Summary:A novel approach combining self-assembly-based colloidal lithography and polydimethylsiloxane (PDMS) micromolding to generate complex protein nanopatterns for studying the mechanisms of leukocyte extravasation within microchannels is presented. Nanostructured surfaces sealed onto PDMS-molded microchannels are chemically functionalized in situ in an all-aqueous process to generate bi-functional chemical nanopatterns. Subsequent co-immobilization with proteins makes use of common non-covalent coupling ( e.g. HIS-tags, FC-tags and biotin-tags), giving nanopatterns of arbitrary combinations of oriented, functional proteins. Up to three different proteins were simultaneously co-immobilized into the microchannel with nanoscale precision, demonstrating the complex patterns. As a proof-of-principle, a mimic of an inflamed endothelium was constructed using a macro- and nanoscale pattern of intercellular adhesion molecule 1 (ICAM1) and P-selectin, and the response of leukocytes through live cell imaging was measured. A clear result on the rolling behavior of the cells was observed with rolling limited to areas where ICAM1 and P-selectin are present. This micro/nano-interface will open new doors to investigations of how spatial distributions of proteins control cellular activity. A nanopatterned microfluidic channel with variable protein compositions and potential use in leukocyte recruitment studies and cancer cell extravasation models is presented.
ISSN:1473-0197
1473-0189
DOI:10.1039/c5lc00916b