An expedient route to highly diversified [1,2,3]triazolo[1,5-a][1,4]benzodiazepines and their evaluation for antimicrobial, antiproliferative and in silico studiesElectronic supplementary information (ESI) available. CCDC 1036732. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5ra12497b

• Main Authors: Sudhapriya, N, Nandakumar, A, Arun, Y, Perumal, P. T, Balachandran, C, Emi, Nobuhiko
• Format: Article
• Language: English
• ISSN: 2046-2069
• DOI: 10.1039/c5ra12497b

An efficient diversity oriented synthesis of [1,2,3]triazolo[1,5- a ][1,4]benzodiazepines has been developed by sequential diazotization, azidation and cycloaddition reactions in a one-pot fashion. This strategy allows an easy accessibility of triazole fused [1,4]benzodiazepines in good yields. The main objective of this methodology is to introduce various substituents at all possible positions under mild reaction conditions. All the synthesized compounds were evaluated for their antimicrobial, anticancer and in silico activity. Among the tested compounds ( 2a-n ), the derivatives 2a , 2b , 2d , 2k , 2g , 2j , 2m and 2l have displayed a broad spectrum of antibacterial activity. Anticancer activity results revealed that compounds 2a , 2g and 2m exhibited potent in vitro anticancer activity against A549 lung adenocarcinoma cancer cell line. Further, molecular docking studies of all the synthesized compounds were performed to gain a comprehensive understanding of the plausible binding modes and also to compare the theoretical and experimental results of these compounds. A simple and facile synthesis of a series of diversified [1,2,3]triazolo[1,5- a ][1,4]benzodiazepines has been achieved successfully via a one-pot method under milder conditions and evaluated for their biological activity.