Photostable AIE fluorogens for accurate and sensitive detection of S-phase DNA synthesis and cell proliferationElectronic supplementary information (ESI) available: Synthetic route and characterization of Cy-Py-N3; PL spectra of TPE-Py-N3 and Cy-Py-N3; flow cytometry of TPE-Py-N3 and Cy-Py-N3; MTT assay and photostability of TPE-Py-N3 and Cy-Py-N3. See DOI: 10.1039/c5tb00458f

Two azide-functionalized tetraphenylethene derivatives with AIE features are synthesized and used as fluorescent agents for detecting S-phase DNA synthesis and cell proliferation based on EdU assay. Compared to the Alexa-azide dye, a commercial DNA bioprobe, the AIE fluorogens show better photostabi...

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Main Authors: Zhao, Yueyue, Yu, Chris Y. Y, Kwok, Ryan T. K, Chen, Yilong, Chen, Sijie, Lam, Jacky W. Y, Tang, Ben Zhong
Format: Article
Language:English
Online Access:Get full text
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Summary:Two azide-functionalized tetraphenylethene derivatives with AIE features are synthesized and used as fluorescent agents for detecting S-phase DNA synthesis and cell proliferation based on EdU assay. Compared to the Alexa-azide dye, a commercial DNA bioprobe, the AIE fluorogens show better photostability and sensitivity, making them promising alternatives. Two azide-functionalized AIE fluorogens are synthesized and used for detecting S-phase DNA synthesis and cell proliferation based on EdU assay.
ISSN:2050-750X
2050-7518
DOI:10.1039/c5tb00458f