High-efficiency immunoassay platforms with controllable surface roughness and oriented antibody immobilizationElectronic supplementary information (ESI) available: Details of synthesis of silica particles, fabrication of the single and dual-scale structured surfaces, preparation of the AAPTS- and CPBA-functionalized surfaces, the oriented immobilization of HSA-Ab, the recognition of HSA-Ag, water contact angle data, AFM images, FTIR-ATR spectra, high-resolution B1s spectra, representative fluore

High-efficiency immunoassay platforms with controlled surface roughness (single- and dual-scale structured surface) were prepared by combining a facile layer-by-layer particle deposition approach with oriented immobilization of antibodies through boronic acid moieties. The as-prepared surfaces showe...

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Main Authors: Song, Lingjie, Zhao, Jie, Luan, Shifang, Ma, Jiao, Ming, Weihua, Yin, Jinghua
Format: Article
Language:English
Online Access:Get full text
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Summary:High-efficiency immunoassay platforms with controlled surface roughness (single- and dual-scale structured surface) were prepared by combining a facile layer-by-layer particle deposition approach with oriented immobilization of antibodies through boronic acid moieties. The as-prepared surfaces showed significantly enhanced antibody loading capacity and antigen recognition, as proved by fluorescence images. High-efficiency immunoassay platforms were facilely prepared by combining a layer-by-layer particle deposition with site-specific antibody immobilization through boronic acid moieties.
ISSN:2050-750X
2050-7518
DOI:10.1039/c5tb01164g