Highly solvatochromic and tunable fluorophores based on a 4,5-quinolimide scaffold: novel CDK5 probes

Novel 4,5-quinolimide-based fluorophores are more solvatochromic and red-shifted than known naphthalimide analogues. Conjugation of one of these fluorophores to a peptide derived from CDK5 kinase demonstrated its sensitivity for monitoring the interaction with its regulatory partner p25. Introductio...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2016-08, Vol.52 (62), p.9652-9655
Main Authors: González-Vera, Juan A, Fueyo-González, Francisco, Alkorta, Ibon, Peyressatre, Marion, Morris, May C, Herranz, Rosario
Format: Article
Language:English
Subjects:
Biochemistry, Molecular Biology
Chemical compounds
Chemical Sciences
Conjugation
Cyclin-Dependent Kinase 5 - chemistry
Fluorescence
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Government regulations
Humans
Kinases
Life Sciences
Medicinal Chemistry
Molecular Structure
Monitoring
Peptides
Pharmaceutical sciences
Quinolines - chemical synthesis
Quinolines - chemistry
Scaffolds
Solvents - chemistry
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Summary:Novel 4,5-quinolimide-based fluorophores are more solvatochromic and red-shifted than known naphthalimide analogues. Conjugation of one of these fluorophores to a peptide derived from CDK5 kinase demonstrated its sensitivity for monitoring the interaction with its regulatory partner p25. Introduction of the quinolimide-labelled peptide into living glioblastoma cells probed the interaction with endogenous p25. Novel 4,5-quinolimide-based solvatochromic fluorophores conjugated to a CDK5 derived peptide demonstrated sensitivity for monitoring CDK5/p25 interaction in living cells.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc04566a