3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells,...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (67), p.1237-124
Main Authors: Pan, Bin-Bin, Yang, Feng, Ye, Yansheng, Wu, Qiong, Li, Conggang, Huber, Thomas, Su, Xun-Cheng
Format: Article
Language:English
Subjects:
Cells (biology)
Dynamics
Marking
Mathematical analysis
NMR spectroscopy
Proteins
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Summary:Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes. The integration of site-specific labeling of proteins with a stable lanthanide binding tag, paramagnetic NMR spectroscopy and the GPS-Rosetta program presents an effective and fast way of determining the three-dimensional structure of a protein in living cells.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc05490k