Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphateElectronic supplementary information (ESI) available. See DOI: 10.1039/c6cc05796a

Enzymatic synthesis and the reverse transcription of RNAs containing 2′- O -carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′- O -carbamoyl uridine triphosphate (U cm TP). U cm TP was incorporated correctly into long RNAs, and its fidelity during reve...

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Main Authors: Masaki, Yoshiaki, Ito, Hyugo, Oda, Yuki, Yamazaki, Kazufumi, Tago, Nobuhiro, Ohno, Kentaro, Ishii, Nozomi, Tsunoda, Hirosuke, Kanamori, Takashi, Ohkubo, Akihiro, Sekine, Mitsuo, Seio, Kohji
Format: Article
Language:English
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Summary:Enzymatic synthesis and the reverse transcription of RNAs containing 2′- O -carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2′- O -carbamoyl uridine triphosphate (U cm TP). U cm TP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection. Enzymatic synthesis and the reverse transcription of RNAs containing 2′- O -carbamoyl uridine were evaluated.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc05796a