Guanidiniocarbonyl pyrrole (GCP) conjugated PAMAM-G2, a highly efficient vector for gene delivery: the importance of DNA condensationElectronic supplementary information (ESI) available. See DOI: 10.1039/c6cc06404c

A novel hybrid compound 1 efficiently shuttles genetic material into HeLa cells at concentrations as low as 0.6 μM, whereas the parent compound PAMAM-G2 is ineffective even at 200 μM. The high efficiency of 1 stems from its capabiliy to form highly condensed ligand-DNA polyplexes. Its binding affini...

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Bibliographic Details
Main Authors: Samanta, Krishnananda, Jana, Poulami, Bäcker, Sandra, Knauer, Shirley, Schmuck, Carsten
Format: Article
Language:English
Online Access:Get full text
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Summary:A novel hybrid compound 1 efficiently shuttles genetic material into HeLa cells at concentrations as low as 0.6 μM, whereas the parent compound PAMAM-G2 is ineffective even at 200 μM. The high efficiency of 1 stems from its capabiliy to form highly condensed ligand-DNA polyplexes. Its binding affinity is actually lower than for the parent dendrimer. Compound 1 is even 200 times more effective and less cytotoxic than PEI, the current gold standard in gene transfection with cationic polymers. A novel hybrid compound 1 efficiently shuttles genetic material into HeLa cells at concentrations as low as 0.6 μM, whereas the parent compound PAMAM-G2 is ineffective even at 200 μM.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc06404c