A chemical reporter strategy for detecting and identifying O-mycoloylated proteins in CorynebacteriumElectronic supplementary information (ESI) available: Experimental details and supplementary data. See DOI: 10.1039/c6cc07143k

Protein O -mycoloylation is a unique post-translational lipidation that was recently discovered in Corynebacterium . We describe an alkyne-modified trehalose monomycolate chemical reporter that can metabolically tag O -mycoloylated proteins in C. glutamicum , enabling their detection and identificat...

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Bibliographic Details
Main Authors: Kavunja, Herbert W, Piligian, Brent F, Fiolek, Taylor J, Foley, Hannah N, Nathan, Temitope O, Swarts, Benjamin M
Format: Article
Language:English
Online Access:Get full text
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Summary:Protein O -mycoloylation is a unique post-translational lipidation that was recently discovered in Corynebacterium . We describe an alkyne-modified trehalose monomycolate chemical reporter that can metabolically tag O -mycoloylated proteins in C. glutamicum , enabling their detection and identification through click chemistry. A trehalose monomycolate (TMM)-mimicking chemical reporter facilitated the investigation of a recently discovered class of lipidated proteins in the Corynebacterineae .
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc07143k