High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosisElectronic supplementary information (ESI) available: Cell culture and detailed sample preparation methods and additional NanoSIMS images. See DOI: 10.1039/c6cc08549k

Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively se...

Full description

Saved in:
Bibliographic Details
Main Authors: Jiang, Haibo, Passarelli, Melissa K, Munro, Peter M. G, Kilburn, Matt R, West, Andrew, Dollery, Colin T, Gilmore, Ian S, Rakowska, Paulina D
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs. Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc08549k