Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogenElectronic supplementary information (ESI) available: Experimental details, FAIM setup, dye cytotoxicity, concentration-dependent fluorescence, and colocalisation imaging. See DOI: 10.1039/c6cc09916e

We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by...

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Bibliographic Details
Main Authors: Soleimaninejad, Hamid, Chen, Moore Z, Lou, Xiaoding, Smith, Trevor A, Hong, Yuning
Format: Article
Language:English
Online Access:Get full text
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Summary:We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively. We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc09916e