Super RLuc8-sFv; a new luciferase-labeled probe for detection of human CD4+ cellsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6mb00652c

The human immunodeficiency virus (HIV) destroys CD4+ lymphocytes and monitoring these cells is one of the best techniques for studying HIV infection. In the present study a novel bioluminescent probe, super RLuc8-sFv, is developed in order to detect human CD4+ cells by fusion of an anti-human CD4 sF...

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Bibliographic Details
Main Authors: Safavi, A, Emamzadeh, R, Nazari, M, Ehsani, M, Zarkesh-Esfahani, S. H, Rahgozar, S
Format: Article
Language:English
Online Access:Get full text
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Summary:The human immunodeficiency virus (HIV) destroys CD4+ lymphocytes and monitoring these cells is one of the best techniques for studying HIV infection. In the present study a novel bioluminescent probe, super RLuc8-sFv, is developed in order to detect human CD4+ cells by fusion of an anti-human CD4 sFv to the C-terminus of super RLuc8. The results indicate that the probe can bind to CD4+ cells via its sFv domain; also it emits visible light through its signalling domain. Super RLuc8-sFv provides a new gateway for detection of human CD4+ cells using luminometric-based assays and may reduce the difficulties involved in, and the cost of, HIV-related diagnostic tests. A probe has been designed, produced and assayed for the detection of T-helper cells. The sFv fragment fused to the C-terminus of super Renilla luciferase 8 (Super RLuc8) and the probe successfully used for detection of human CD4+ cells using luminometer.
ISSN:1742-206X
1742-2051
DOI:10.1039/c6mb00652c